Lectures and seminars Seminar: "Coordinated remodeling of neural stem cell epigenome and cell fate bias during cortical development"
Welcome to a seminar with Dr. Boyan Bonev, Helmholtz Zentrum München, Germany.
Title
"Coordinated remodeling of neural stem cell epigenome and cell fate bias during cortical development"
The long-term objective of the Bonev lab is to decode the genetic and epigenetic blueprints underlying brain development, evolution, and disease. To accomplish this, they study how the interplay between transcription factors, 3D genome organization, and gene expression contribute to cell fate decisions. Their approach combines developmental neurobiology, single-cell omics, mouse genetics, CRISPR-based perturbations, and computational biology to unravel complex regulatory networks. A distinctive aspect of the group’s work is the development of innovative tools for simultaneous multimodal epigenome profiling. By harnessing single-cell multiomic technologies and applying massively parallel reporter assays in vivo and in human brain organoids, they capture the dynamic interplay among chromatin accessibility, DNA methylation, and nuclear architecture across neuronal lineages, pinpointing the regulatory elements that influence neuronal phenotypes in both health and disease.
Speaker
Dr. Boyan Bonev, Helmholtz Zentrum München, Germany.
Selected publications
A. Pereira, J. Diwakar, T. Simon, Y. So, LM Loarte, F. Bergmann, T. Schauer, G. Masserdotti, A. Danese, R. Bocchi, M. Colomé-Tatché, A. Philpott, T. Straub, Bonev B#, Götz M# Direct neuronal reprogramming of mouse astrocytes is associated with multiscale epigenome remodeling and requires Yy1. Nature Neuroscience (2024) 27(7):1260-1273
Noack F.*, Vangelisti S.*, Bölicke, N., Diwakar J., Chong F. Albert, M. & Bonev B Joint epigenome profiling reveals cell-type-specific gene regulatory programs in human cortical organoids. Nature Cell Biology (2023) 25, 1873–1883
Noack F., Vangelisti S., Raffl G., Carido M., Diwakar J., Chong F. & Bonev B Multimodal profiling of the transcriptional regulatory landscape of the developing mouse cortex identifies Neurog2 as a key epigenome remodeler. Nature Neuroscience (2022) 25, 154-167.
Host
Gonçalo Castelo-Branco, Department of Medical Biochemistry and BIophysics, KI