Solving the puzzle pieces of the mitochondrial ribosome assembly

Human cells contain two types of ribosomes, the cell’s so-called protein factories. One exists in the cytosol, the other in the mitochondria. In mitochondria, mitoribosomes synthetize the small subset of proteins responsible for oxidative phosphorylation and energy production. Their dysregulation contributes to complex pathologies, including neurodegenerative diseases, such as Alzheimer’s and Parkinson’s disease.

Building mitoribosomes requires coordinated action of multiple protein factors

The synthesis of ribosomes is an extremely complex, multistep process, which includes both ribosomal RNA (rRNA) folding and the ordered association of dozens of accessory proteins to the growing ribosome. This complexity explains why many of the details of the process are incompletely understood.

In bacteria, who are hypothesized to share evolutionary history with mitochondria, several GTP-binding proteins have been shown to be involved in ribosome assembly.

Two human homologues of the bacterial assembly factor ObgE have been found to reside in human mitochondria, GTPBP10 and GTPBP5. The function of GTPBP10 has previously been investigated by the authors of this study, revealing it to be involved in the assembly of the mitoribosomal large subunit.  In the current study, the researchers investigate GTPBP5. Using proteomic and biochemical analyses, they show that GTPBP5 interacts with several previously characterized assembly factors during latest stages of mitoribosome production. Loss of the factor results in impaired mitochondrial translation, making its function of fundamental importance to cellular metabolism.

“Understanding the molecular basis underlying complex pathways such as mitochondrial biogenesis is a great challenge. GTPBP5 is only one of many factors implicated in this process and we have only just begun to scratch the surface.” says Miriam Cipullo, author and PhD student in the group.

“Our study provides insight into the molecular mechanisms of mitoribosome biogenesis and places GTPBP5 amongst candidate disease genes in patients with OxPhos and other metabolic deficiencies” says Joanna Rorbach, Assistant Professor at the Department of Medical Biochemistry and Biophysics at Karolinska Institutet. 

The study was supported by the Max Planck Society; Karolinska Institutet; Knut and Alice Wallenberg Foundation; EU Marie Sklodowska Curie Actions.

Publication

Human GTPBP5 is involved in the late stage of mitoribosome large subunit assembly

Miriam Cipullo, Sarah F. Pearce, Isabel G. Lopez Sanchez, Shreekara Gopalakrishna, Annika Krüger, Jakob D. Busch, Xinping Li, Ilian Atanassov, Joanna Rorbach. DOI: gkaa1131