Lectures and seminars Infection and Immunology Seminar: "Characterisation of Bacillus spores"

Title

"Characterisation of Bacillus spores"

Host

Ute Römling, Department of Microbiology, Tumor and Cell Biology, KI

Speaker

Prof. Marina Aspholm, Norwegian University of Life Sciences, Department of Paraclinical Sciences, Faculty of Veterinary Medicine, Ås, Norway.

Summary:

Prof. Aspholm’s research deals with the analysis and ecological relevance of endospore appendages.

1: Zegeye ED, Sleutel M, Jonsmoen UL, Chen J, Morawska LP, Mekonnen YB, Kuipers

OP, Remaut H, Aspholm M. Spatiotemporal expression of endospore appendages and

cryo-EM insights into Ena1C-mediated S-ENA anchoring in Bacillus paranthracis.

Sci Rep. 2026 Feb 3;16(1):7122. doi: 10.1038/s41598-026-38321-0. PMID: 41634327;

PMCID: PMC12921279.

2: Albertsdottir Jonsmoen UL, Allred JA, Malyshev D, Segervald J, Andersson M,

Aspholm ME. Endospore appendages enhance adhesion of <i>Bacillus cereus sensu

lato</i> spores to industrial surfaces, modulated by physicochemical factors.

Appl Environ Microbiol. 2025 Nov 19;91(11):e0094425. doi: 10.1128/aem.00944-25.

Epub 2025 Oct 7. PMID: 41055375; PMCID: PMC12628813.

3: Sleutel M, Sogues A, Van Gerven N, Jonsmoen UL, Aspholm M, Van Molle I,

Fislage M, Theunissen L, Bellis N, Baquero D, Egelman E, Krupovic M, Wang J,

Remaut H. Cryo-EM analysis of the Bacillus thuringiensis extrasporal matrix

identifies F-ENA as a widespread family of endospore appendages across

Firmicutes. Res Sq [Preprint]. 2025 Mar 13:rs.3.rs-6050303. doi:

10.21203/rs.3.rs-6050303/v1. Update in: Nat Commun. 2025 Aug 16;16(1):7652. doi:

10.1038/s41467-025-62896-3. PMID: 40162231; PMCID: PMC11952670.

4: Sleutel M, Sogues A, Van Gerven N, Jonsmoen UL, Van Molle I, Fislage M,

Theunissen LD, Bellis NF, Baquero DP, Egelman EH, Krupovic M, Wang F, Aspholm M,

Remaut H. Cryo-EM identifies F-ENA of Bacillus thuringiensis as a widespread

family of endospore appendages across Firmicutes. Nat Commun. 2025 Aug

16;16(1):7652. doi: 10.1038/s41467-025-62896-3. PMID: 40818982; PMCID:

PMC12357932.

5: Jonsmoen UL, Malyshev D, Sleutel M, Kristensen EE, Zegeye ED, Remaut H,

Andersson M, Aspholm ME. The role of endospore appendages in spore-spore

interactions in the pathogenic Bacillus cereus group. Environ Microbiol. 2024

Sep;26(9):e16678. doi: 10.1111/1462-2920.16678. PMID: 39228067.

6: Jonsmoen UL, Malyshev D, Öberg R, Dahlberg T, Aspholm ME, Andersson M.

Endospore pili: Flexible, stiff, and sticky nanofibers. Biophys J. 2023 Jul

11;122(13):2696-2706. doi: 10.1016/j.bpj.2023.05.024. Epub 2023 May 22. PMID:

37218131; PMCID: PMC10397575.

7: Zegeye ED, Pradhan B, Llarena AK, Aspholm M. Enigmatic Pilus-Like Endospore

Appendages of <i>Bacillus cereus</i> Group Species. Int J Mol Sci. 2021 Nov

16;22(22):12367. doi: 10.3390/ijms222212367. PMID: 34830248; PMCID: PMC8619143.

8: Pradhan B, Liedtke J, Sleutel M, Lindbäck T, Zegeye ED, O Sullivan K, Llarena

AK, Brynildsrud O, Aspholm M, Remaut H. Endospore Appendages: a novel pilus

superfamily from the endospores of pathogenic Bacilli. EMBO J. 2021 Sep

1;40(17):e106887. doi: 10.15252/embj.2020106887. Epub 2021 May 25. PMID:

34031903; PMCID: PMC8408608.