Other Workshop 1: Human iPS cell gene editing - with a focus on knock-out

29-09-2025 9:00 am to
03-10-2025 4:00 pm Add to iCal
Campus Flemingsberg KISCO Facility at the Department of Laboratory Medicine, Karolinska Institutet

Learn How to Master Gene Editing!
Are you new to gene editing? Or would like to acquire hands on experience and analytical skills to conduct gene editing?

Join our comprehensive 5-day course covering the full process of gene editing, from experimental design to post-edit quality control. Learn the latest methods and techniques from leading experts and gain hands-on experience in gene-editing applications.

Who Should Attend?

This course is designed for anyone new to gene editing, including technical assistantsPhD students, and principal investigators (PIs) looking to build their expertise.

Please note: Participants will receive a certificate upon completion. While applicants from all universities are welcome, priority is given to local participants.

Why Join?

  • Learn practical aspects of cutting-edge gene-editing techniques from experts
  • Gain hands-on experience in experimental design and data analysis
  • Network with researchers and professionals in the field
  • Receive a certificate of completion

Course Highlights

  • Designing sgRNA and crRNA with high activity
  • Cloning of sgRNA and crRNA in dual expression systems. Assembly of RNP complexes.
  • Electroporation based nuclease delivery
  • Evaluating edits in bulk populations and clonal situations
  • Post-edit quality control and workflow reporting
  • Experimental design, data analysis, and result interpretation
  • Current CRISPR toolbox and its applications
  • CRISPRa/i and advanced editing techniques
  • Prime editing and base editing
  • CRISPR screening strategies
  • Designing gene-editing experiments
  • Cloning of sgRNAs
  • Electroporation delivery into human iPS cells.
  • Analysing bulk-edited cells
  • Evaluating individual clones

Expert Instructors

  • Experts from Karolinska Instititutet Stem Cells & Organoids Core Facility (KISCO)
  • Dr. Jonathan Arias

Course fee

  • 2,000 EUR for national academic participants
  • For external customers: Contact us for availability and pricing

Contact

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Jose Inzunza

Director of the KISCO facility
Department of Laboratory Medicine
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Jonathan Arias

Expert in Gene-Editing- Technology