Lectures and seminars Webinar: PrimeFlow RNA Assay- Simultaneous detection of RNA and protein by flow cytometry

04-03-2024 2:30 pm - 3:30 pm Add to iCal
Online

Microarrays and sequencing provide comprehensive gene expression data in bulk sample preparations; however, the analysis of bulk samples can mask the individual effects of unique cellular subsets. Coupling RNA expression with protein detection on a flow cytometer generates multiparametric data in heterogeneous cell populations and offers in-depth and high-content details at the single-cell level.

The PrimeFlow RNA assay employs fluorescence in situ hybridization (FISH) with branched DNA (bDNA) signal amplification for the simultaneous detection of up to four RNA targets, and it can be used in combination with immunolabeling of both cell-surface and intracellular proteins using fluorophore-conjugated antibodies.

Date & Time

4 March at 14:30-15:30

Speaker

Jaschke Margit, Technical application scientist, EMEA

Contact

Alda Saldan: alda.saldan@ki.se
Marina Polo Gozalo: marina.polo.gozalo@ki.se

Hosted by: Thermo Fisher Scientific in collaboration with Biomedicum, Flow Core Facility, Karolinska Institutet, Stockholm, Sweden

Teams link

Join the webinar here

Meeting ID: 219 492 648 946 

Passcode: 5vuQmN

 

Contact

Alda Saldan Research Infrastructure Specialist