Lectures and seminars Spectral Flow Cytometry Seminar

Title

Spectral flow cytometry - "Leveraging novel fluorophore technologies to enable flexible and higher dimensional immune profiling by spectral flow cytometry."

Learning Objectives

• Introduction to Spectral flow cytometry.
• Advantages of spectral flow cytometry over conventional flow cytometry.
• How NovaFluors could help you in expansion of the panel.

Speaker

Seddon Y. Thomas, Ph.D., Staff Scientist, R&D, Thermo Fisher Scientific, Durham, North Carolina USA​.

Biography

Seddon Y. Thomas, Ph.D. joined Thermo Fisher Scientific in 2020 as a R&D Staff Scientist, bringing over 20 years of experience in flow cytometry and immunology research. Seddon was previously a Research Application Scientist at Phitonex, that pushed the boundaries of fluorescence labeling by incorporating DNA scaffolding into novel reporters for high dimensional flow cytometry panels run with 40 and 45 colors. '

Seddon earned her B.A. in Chemistry from Williams College and her Ph.D. in Medical Sciences in Immunology and Virology from Harvard Medical School. During her graduate work at Harvard and postdoctoral training at the University of Chicago and the NIH, she published 35 papers and received numerous honors including the AAI Young Investigator Award, President’s Rising Stars in Mucosal Immunology, and multiple NIH FARE awards.      

Abstract

High dimensional full spectrum flow cytometry holds the promise of addressing long-held questions in cellular biology. Fluorescent dye performance heavily impacts both the quality and the dimensionality of flow cytometry experiments. The development of fluorescent labels engineered with targeted excitation and emission spectra using the Invitrogen™ Phiton™ DNA platform has allowed the generation of flow cytometry panels with the goal of reducing spectral spread and increasing biological resolution.

In this tutorial, we will take a deep dive into the data generated using a high parameter panel while leaving available placeholders for commonly used fluorophores. We will also discuss the iterative panel design process and identify problematic fluorophore combinations as well as best practices for panel design practices that enable greater biological resolution.

Discuss your own research?

If you would like to discuss your research or flow cytometry panels one to one with ThermoFisher, please email youssef.elsharabasy@thermofisher.com so he can book some time for you.