Lectures and seminars Seminar: "Long-term in vitro expansion of a human fetal pancreas stem cell that generates all three pancreatic cell lineages"

Title

"Long-term in vitro expansion of a human fetal pancreas stem cell that generates all three pancreatic cell lineages"

Speaker

Dr Andersson-Rolf is currently a postdoc in Dr Hans Clevers' lab at the Hubrecht Institute in the Netherlands, where she investigates stem and differentiated cells in human pancreas development and disease, and developed a human fetal pancreatic organoid model that mimics native epithelial organ complexity by including the three main cell lineages. 

She earned a Master of Medical Biotechnology degree at the Royal Institute of Technology, Sweden, where she established a method for creating an in vitro-generated endogenous organoid reporter line. She then earned a PhD at the WT-MRC Stem Cell Institute, Cambridge University, UK, where she developed advanced genetic tools for interrogating gene function.

Selected publications

Andersson-Rolf A, Groot K, Korving J, Hanegraaf M, Begthel H, van de Wetering WJ, VanInsberghe M, Salmen F, van den Brink S, Lopez-Iglesias C, Peters PJ, de Koning E.J.P, Geurts M.H, Beumer J, Alemany A, Gehart H, Chuva de Sousa Lopes SM, van Oudenaarden A, van Es JH, Clevers H. (2024). Long-term in vitro expansion of a human fetal pancreas stem cell that generates all three pancreatic cell lineages. Cell, online December 2, 2024

Beumer, J., Geurts, M. H., Geurts, V., Andersson-Rolf, A., Akkerman, N., Völlmy, F., Krueger, D., Busslinger, G. A., Martínez-Silgado, A., Boot, C., Yousef Yengej, F. A., Puschhof, J., Knoops, K., López-Iglesias, C., Peters, P. J., Vivié, J. A., Mooijman, D., & Clevers, H. (2024). Description and functional validation of human enteroendocrine cell sensors. Science. 386 (6719), 341-348. 

Andersson-Rolf, A., Mustata, R. C., Merenda, A., Kim, J., Perera, S., Grego, T., Andrews, K., Tremble, K., Silva, J. C., Fink, J., Skarnes, W. C., & Koo, B-K. (2017). One-step generation of conditional and reversible gene knockouts. Nature Methods, 14(3), 287-289. https://doi.org/10.1038/nmeth.4156

Andersson-Rolf, A., Merenda, A., Mustata, R. C., Li, T., Dietmann, S., & Koo, B-K. (2016). Simultaneous paralogue knockout using a CRISPR-concatemer in mouse small intestinal organoids. Developmental Biology, 420(2), 271-277. https://doi.org/10.1016/j.ydbio.2016.10.016

Broutier, L., Andersson-Rolf, A., Hindley, C. J., Boj, S. F., Clevers, H., Koo, B., & Huch, M. (2016). Culture and establishment of self-renewing human and mouse adult liver and pancreas 3D organoids and their genetic manipulation. Nature Protocols, 11(9), 1724-1743. https://doi.org/10.1038/nprot.2016.097

Host

MBB Faculty / MBB Frontier Grant Recruitment