Lectures and seminars "RE-CREATING and SCRIPT: Emerging Technologies for Overcoming Mouse Genome Engineering Challenges"
Welcome to a seminar on 15 September 2025, with Channabasavaiah B. Gurumurthy, Professor at the Department of SOM-Cell and Molecular Biology and Director of Genome-editing Reseearch, Education and Animal Genome Targeting (GREAT), University of Mississipi Medical Center, USA.
Abstract
Over the past decade, our laboratory has led the development of transformative genome engineering technologies, including Easi-CRISPR and i-GONAD which have become widely adopted for creating genetically engineered mouse models across the globe. While these methods have enabled the generation of thousands of models each year, their efficiency drops significantly when working with large DNA inserts—typically those exceeding a few kilobases.
In this presentation, I will introduce two emerging technologies developed in our laboratory that address these limitations: RE-CREATING (RE-engineering using CRISPR of previously Engineered Alleles To Insert New Genes) and SCRIPT (Simultaneous CRISPR and PITT). I will share experimental data demonstrating how these methods enhance the efficiency and accuracy of inserting large DNA fragments, opening new avenues for complex genome engineering in vivo.