Lectures and seminars Infection and Immunology Seminar: "Deciphering modes of action of antimalarial drugs by thermal protein profiling"

Title

"Deciphering Modes of Action of Antimalarial Drugs by Thermal Protein Profiling"

Speaker

Zbynek Bozdech, School of Biological Sciences, Nanyang Technological University, Singapore

Selected Publications

1. Samuel Pazicky, Seth Tjia, Guilherme B. Farias, Nisha Philip, Radoslaw M. Sobota, Andrew P. Waters, Tim-Wolf Gilberger, Zbynek Bozdech, Thermal profiling maps the dynamic interactome in malaria parasites. https://doi.org/10.21203/rs.3.rs-5659511/v1 This is a preprint just accepted by Nature Microbiology for publication.

2. Go, K. D., Yan, X. F., Wirjanata, G., Ero, R., Pazicky, S., Dziekan, J., Tjia, S., Lescar, J., Bozdech, Z. & Gao, Y. G. Antimalarial drug artemisinin stabilizes PfRACK1 binding to the ribosome. Structure 33, 1386-1397 e1385, doi:10.1016/j.str.2025.05.008 (2025).

3. Wirjanata, G., Lin, J., Dziekan, J. M., El Sahili, A., Chung, Z., Tjia, S., Binte Zulkifli, N. E., Boentoro, J., Tham, R., Jia, L. S., Go, K. D., Yu, H., Partridge, A., Olsen, D., Prabhu, N., Sobota, R. M., Nordlund, P., Lescar, J. & Bozdech, Z. Identification of an inhibitory pocket in falcilysin provides a new avenue for malaria drug development. Cell Chem Biol 31, 743-759 e748, doi:10.1016/j.chembiol.2024.03.002 (2024).

4. Dziekan, J. M., Wirjanata, G., Dai, L., Go, K. D., Yu, H., Lim, Y. T., Chen, L., Wang, L. C., Puspita, B., Prabhu, N., Sobota, R. M., Nordlund, P. & Bozdech, Z. Cellular thermal shift assay for the identification of drug-target interactions in the Plasmodium falciparum proteome. Nat Protoc 15, 1881-1921, doi:10.1038/s41596-020-0310-z (2020).

5. Dziekan, J. M., Yu, H., Chen, D., Dai, L., Wirjanata, G., Larsson, A., Prabhu, N., Sobota, R. M., Bozdech, Z. & Nordlund, P. Identifying purine nucleoside phosphorylase as the target of quinine using cellular thermal shift assay. Sci Transl Med 11, doi:10.1126/scitranslmed.aau3174 (2019).

Host

Gerald McInerney, Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet