Lectures and seminars Guest seminar - Jik Nijssen, Max Planck Institute

24-06-2024 10:00 am - 11:00 am Add to iCal
Campus Solna Nils Ringertz, Biomedicum, Solnavägen 9, Karolinska Institutet, Solna

Title: Aggregation of the ALS-related protein TDP-43 is triggered by cellular oxidation and stress granule recruitment.

Welcome to guest seminar by Jik Nijssen, postdoc from Max Planck Institute of Molecular Cell Biology and Geneticsi, Dresden, Germany

Date: June 24th
Time: 10am
Place: Nils Ringertz, Biomedicum 

Aggregates of the TDP-43 protein are the most common hallmark seen in affected neurons in the neurodegenerative disease amyotrophic lateral sclerosis (ALS). TDP-43 is normally a nuclear protein, and it is still unclear what triggers the formation of cytosolic aggregates.

Recently, TDP-43 was shown to localize to a type of biomolecular condensate inside the cytoplasm, termed stress granules. We set out to study the behavior of TDP-43 inside stress granules.

Using in vitro protein reconstitution assays, as well as work in cell lines and stem cell-derived motor neurons, we found that TDP-43 can aggregate from within the stress granule environment under specific cellular stress conditions. We finally established a two-hit model for TDP-43 aggregation, and generated engineered variants of TDP-43 that are resistant to aggregation.

Bio:
I performed my undergrad studies in Neuroscience in Maastricht, the Netherlands, ending with my master’s thesis which was performed at Karolinska in prof. Eva Hedlund’s lab in 2014-2015. I then stayed in Eva’s lab for my PhD (2015-2020), combining stem cells and RNA sequencing, with a focus on studying differences in RNA localization between neuronal cell bodies and their axons. After the PhD I moved to Dresden, Germany to start a post-doc in the lab of prof. Anthony Hyman (2021 – present). Here I study how protein phase separation contributes to aggregation, in the context of ALS and neurodegeneration.

Host: Eva Hedlund

Contact

Eva Hedlund Principal Researcher