Lectures and seminars BFC Flow Cytometry User Group Meeting

“OMIP-112: 42-Parameter (40 Color) Spectral Flow Cytometry Panel for Comprehensive Immunophenotyping of Human Peripheral Blood Leukocytes" 

In this webinar, Laurien Waaijer will describe the development of OMIP-112, a flow cytometry panel that enables the detection of 80 different immune cell subsets in a single measurement. 
 

Among the topics that will be discussed:

•Selection of fluorochromes

•Autofluorescence management

•Antibody titration

•Staining protocols

•Data analysis

Abstract: Profiling the human immune system is essential for understanding its role in health and disease. Using our 42-parameter flow cytometry panel (OMIP-112), we can identify more than 80 unique immune cell subsets in a single measurement. However, developing such a comprehensive panel, presents several challenges that require careful consideration, including antigen–fluorochrome selection, autofluorescence management, antibody titration, staining protocols, and subsequent data analysis. In this presentation, I will discuss these key steps to help you get started with panel design and highlight important considerations for analysing high-dimensional flow cytometry data.

Speaker

Laurien Waaijer, PhD Candidate, Radboud University Medical Center.

Biosketch: Laurien is a PhD student specializing in translational immunology and advanced cytometry. Her research focuses on immune profiling in chronic inflammatory diseases, integrating high-dimensional flow cytometry to dissect immune mechanisms of treatment response. She has contributed to immune monitoring studies, including the development and publication of OMIP-112, and is currently applying this panel in longitudinal cohorts of inflammatory bowel disease and atopic dermatitis. Her work aims to advance precision medicine approaches in the treatment of chronic inflammatory diseases.

Join the seminar via Zoom

Follow the link here to join the meeting

Meeting ID: 626 0700 0859
Passcode: 730219